Assessment of Oxidative DNA Damage by Alkaline Comet Assay in Human Essential Hypertension
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منابع مشابه
Studies on electron beam induced DNA damage and repair kinetics in lymphocytes by alkaline comet assay
Background: Exposure to ionizing radiation is known to induce oxidative stress followed by damage to critical biomolecules like lipids, proteins and DNA through radiolysis of cellular water. Since radiation has been widely used as an important tool in therapy of cancer, the detailed investigation regarding the DNA damage and repair kinetics would help to predict the radiation sensitivity of cel...
متن کاملA modified alkaline Comet assay for in vivo detection of oxidative DNA damage in Drosophila melanogaster.
Modifications to the alkaline Comet assay by using lesion-specific endonucleases, such as formamidopyrimidine-DNA glycosylase (FPG) and endonuclease III (ENDOIII, also known as Nth), can detect DNA bases with oxidative damage. This modified assay can be used to assess the genotoxic/carcinogenic potential of environmental chemicals. The goal of this study was to validate the ability of this modi...
متن کاملstudies on electron beam induced dna damage and repair kinetics in lymphocytes by alkaline comet assay
background: exposure to ionizing radiation is known to induce oxidative stress followed by damage to critical biomolecules like lipids, proteins and dna through radiolysis of cellular water. since radiation has been widely used as an important tool in therapy of cancer, the detailed investigation regarding the dna damage and repair kinetics would help to predict the radiation sensitivity of cel...
متن کاملAn ECVAG† trial on assessment of oxidative damage to DNA measured by the comet assay
The increasing use of single cell gel electrophoresis (the comet assay) highlights its popularity as a method for detecting DNA damage, including the use of enzymes for assessment of oxidatively damaged DNA. However, comparison of DNA damage levels between laboratories can be difficult due to differences in assay protocols (e.g. lysis conditions, enzyme treatment, the duration of the alkaline t...
متن کاملDNA damage by ochratoxin A in rat kidney assessed by the alkaline comet assay.
There are few studies of ochratoxin A (OTA) genotoxicity in experimental animals and the results obtained with cell cultures are inconsistent, although the carcinogenic potential of OTA for the kidney of experimental animals has been well established. We studied the genotoxic potential of OTA in the kidney of adult female Wistar rats (5 in each group) treated intraperitoneally with OTA (0.5 mg ...
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ژورنال
عنوان ژورنال: Indian Journal of Clinical Biochemistry
سال: 2015
ISSN: 0970-1915,0974-0422
DOI: 10.1007/s12291-015-0521-1